Detection, Quantification, and Microlocalisation of Targets of Pesticides Using Microchannel Plate Autoradiographic Imagers

Citation

Tarhoni, M.H.; Vigneswara, V.; Smith, M.; Anderson, S.; Wigmore, P.; Lees, J.E.; Ray, D.E.; Carter, W.G. Detection, Quantification, and Microlocalisation of Targets of Pesticides Using Microchannel Plate Autoradiographic Imagers. Molecules 2011, 16, 8535-8551.

  • microchannel plate detector
  • molecular imaging
  • pesticides
  • neurotoxicity
  • immunotoxicity
  • proteomics
  • post-translational modifications
  • exposure biomarkers

Brief

Microchannel plate (MCP) autoradiographic imaging is a highly sensitive method for detecting and quantifying protein adduction by pesticides.

Summary

Organophosphorus (OP) pesticides can bind to proteins in addition to acetylcholinesterase (AChE), their primary target.

A study used a microchannel plate (MCP) autoradiographic detector to monitor a radiolabeled OP tracer compound to identify and quantify OP pesticide protein binding targets in rat thymus tissue. The researchers found that the OP pesticides chlorfenvinphos-oxon, chlorpyrifos-oxon, and diazinon-oxon significantly reduced the radiolabeling of the 59 kDa protein target. The same three pesticides also significantly reduced radiolabeling of the 66 kDa protein target. Only chlorpyrifos-oxon and diazinon-oxon significantly reduced the radiolabeling of the 82 kDa protein target. The researchers note that these findings suggest that these commonly used pesticides do not exhibit identical binding characteristics to protein targets. Therefore, their structure-activity relationships will need to be evaluated on an individual compound basis.

Origin: https://www.semanticscholar.org/reader/d65dd8c8f835f9703cb80914dddfb6f6dd6af103

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