Multicolor fluorescence microscopy for surgical guidance using a chip-scale imager with a low-NA fiber optic plate and a multi-bandpass interference filter
Citation
Roschelle, M., Rabbani, R., Papageorgiou, E., Zhang, H., Cooperberg, M., Stohr, B. A., ... & Anwar, M. (2024). Multicolor fluorescence microscopy for surgical guidance using a chip-scale imager with a low-NA fiber optic plate and a multi-bandpass interference filter. Biomedical Optics Express, 15(3), 1761–1776.
Keywords
- Fluorescence-guided surgery (FGS)
- VISION
- intraoperative imaging
- multicolor fluorescence microscopy
- cancer surgery
- microscopic tumor foci
- nerve visualization
- lens-less imaging
- CMOS image sensor
- fiber optic plate (FOP)
- interference filter
Brief
VISION is a scalable, lens-less fluorescence imaging chip that is capable of sensitive and multiplexed detection within a compact form factor.
Summary
This article presents VISION, a versatile lens-less, chip-based fluorescence imaging platform for surgical guidance. VISION addresses limitations of conventional fluorescence-guided surgery (FGS) systems, which are often bulky, rigid, and limited to single-target imaging, hindering their use for detecting microscopic disease and distinguishing between healthy and diseased tissue during surgery.
VISION's contact imaging approach, where the image sensor directly contacts the tissue, eliminates the need for bulky lenses and enables high-resolution, multiplexed imaging within a compact form factor. The system uses a low numerical aperture fiber optic plate (LNA-FOP) and a multi-bandpass interference filter to improve resolution and enable multicolor imaging.
The article demonstrates VISION's ability to detect tumor foci smaller than 100 cells at near video frame rates. Ex vivo imaging of resected prostate tissue shows the system's potential for identifying microscopic disease at resection margins, differentiating tumors from nerves, and assessing tumor spread, highlighting its potential clinical significance in cancer surgery.
Origin: https://opg.optica.org/directpdfaccess/51997303-f8e4-4ad2-bb1da245a7640fd5_547033/boe-15-3-1761.pdf?da=1&id=547033&seq=0&mobile=no