Miniaturized LED light source with an excitation filter for fluorescent imaging

Miniaturized LED light source with an excitation filter for fluorescent imaging

Citation

Azmer, M. I., Sasagawa, K., Rustami, E., Sugie, K., Ohta, Y., Haruta, M., ... & Ohta, J. (2021). Miniaturized LED light source with an excitation filter for fluorescent imaging. Japanese Journal of Applied Physics, 60(SBBG07), SBBG07. This citation uses the authors listed in source, the article title from source, the journal title, volume, and issue from source, and the page range from source.

  • Miniaturized LED light source
  • Excitation Filter
  • Fluorescent Imaging
  • Implantable Lens-Less Brain Imaging System
  • InGaN Blue µLED
  • Laser Lift-Off (LLO)
  • Fiber Optic Plate (FOP)
  • Green Fluorescent Proteins (GFPs)
  • CMOS Image Sensor

Brief

Researchers developed an implantable device to capture brain images in mice using a miniaturized LED light source and a filter to isolate green fluorescent protein emissions.

Summary

This 2021 article by M. I. Azmer et al., published in the Japanese Journal of Applied Physics, details the development of a miniaturized InGaN blue μLED-based excitation source for an implantable, lens-less brain imaging system. The primary goal of this research was to create a device thin enough for implantation while still powerful enough to produce bright fluorescence images for in vivo applications.
To achieve this, researchers:
  • Used a laser lift-off process to thin the μLED for improved implantability.
  • Integrated a filtering system comprising a short-pass interference filter and a low-NA fiber optic plate (FOP) to reduce undesired light emissions from the μLED.
  • Tested the device's imaging capabilities on a mouse brain slice genetically modified to express green fluorescent protein (GFP).

The resulting device was only 180 μm thick, making it suitable for implantation. The researchers successfully demonstrated its effectiveness by capturing bright fluorescence images of the brain slice, suggesting its potential for in vivo brain imaging.

Origin: https://iopscience.iop.org/article/10.35848/1347-4065/abe5bf
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